In syringe hybrid monoliths modified with gold nanoparticles for selective extraction of glutathione in biological fluids prior to its determination by HPLC.

6533b82ffe1ef96bd129515d

RESEARCH PRODUCT

In syringe hybrid monoliths modified with gold nanoparticles for selective extraction of glutathione in biological fluids prior to its determination by HPLC.

María Vergara-barberán Ernesto F. Simó-alfonso José Manuel Herrero-martínez ÓScar Mompó-roselló

subject

Glycidyl methacrylate Metal Nanoparticles 02 engineering and technology 01 natural sciences Analytical Chemistry chemistry.chemical_compound Limit of Detection Humans Solid phase extraction Monolith Derivatization Saliva Chromatography High Pressure Liquid Detection limit geography geography.geographical_feature_category Chromatography Syringes 010401 analytical chemistry Extraction (chemistry)Solid Phase Extraction Reproducibility of Results 021001 nanoscience & nanotechnology Glutathione 0104 chemical sciences chemistry Colloidal gold Epoxy Compounds Methacrylates Gold 0210 nano-technology Hybrid material

description

Abstract In this work, a simple device for extraction glutathione (GSH) in biological fluids using a hybrid monolithic material within a polypropylene syringe is developed. For this purpose, glycidyl methacrylate-based monolith was firstly prepared within this housing material, and the polymer was modified with different ligands (ammonia, cysteamine and cystamine). The resulting materials (containing amine or thiol groups, respectively) were then functionalized with gold nanoparticles (AuNPs). The hybrid material that gave the largest AuNPs coverage was selected as solid-phase (SPE) sorbent and several variables affecting the extraction recovery of this compound were investigated. Under optimal conditions, GSH was quantitatively retained at pH 6.0, and then it was desorbed with aqueous dithiothreitol solution and determined, after derivatization with o-phthaldialdehyde, via reversed-phase LC with fluorometric detection. The limit of detection was ca. 1.5 ng mL−1, and the reproducibility between extraction units was below 8% (expressed as relative standard deviation), which demonstrates the robustness of the method. The developed material was also applied for the extraction of GSH in saliva and urine samples yielding recoveries ranging from 86 to 105%.

year	journal	country	edition	language
2020-03-01	Talanta

10.1016/j.talanta.2019.120566 https://pubmed.ncbi.nlm.nih.gov/31892018