6533b82ffe1ef96bd129537b

RESEARCH PRODUCT

bilbo, a non-LTR retrotransposon of Drosophila subobscura: a clue to the evolution of LINE-like elements in Drosophila

M J Martínez-sebastiánDavid Blesa

subject

Transposable elementUntranslated regionRetroelementsSequence analysisvirusesMolecular Sequence DataRetrotransposonBiologyPolymerase Chain ReactionHomology (biology)Evolution MolecularGeneticsAnimalsAmino Acid SequenceCloning MolecularMolecular BiologyPhylogenyEcology Evolution Behavior and SystematicsDNA PrimersRepetitive Sequences Nucleic AcidGeneticsBase SequenceSequence Homology Amino AcidPhylogenetic treeDrosophila subobscuraOpen reading frameDrosophila

description

We used the repetitive character of transposable elements to isolate a non-LTR retrotransposon in Drosophila subobscura. bilbo, as we have called it, has homology to TRIM and LOA elements. Sequence analysis showed a 5' untranslated region (UTR), an open reading frame (ORF) with no RNA-binding domains, a downstream ORF that had structural homology to that of the I factor, and, finally, a 3' UTR which ended in several 5-nt repeats. The results of our phylogenetic and structural analyses shed light on the evolution of Drosophila non-LTR retrotransposons and support the hypothesis that an ancestor of these elements was structurally complex.

yearjournalcountryeditionlanguage
1997-11-19Molecular Biology and Evolution
https://doi.org/10.1093/oxfordjournals.molbev.a025724