6533b82ffe1ef96bd129539c

RESEARCH PRODUCT

Phylogenetic analysis of the isopenicillin-N-synthetase horizontal gene transfer.

Andrés MoyaCelia Buades

subject

Time FactorsSequence alignmentGram-Positive BacteriaAspergillus nidulansFungal ProteinsTransformation GeneticBacterial ProteinsSpecies SpecificityPhylogeneticsAspergillus nidulansBotanyGram-Negative BacteriaGeneticsMolecular clockMolecular BiologyGeneEcology Evolution Behavior and SystematicsPhylogenyGeneticsFungal proteinLikelihood FunctionsbiologyPhylogenetic treeModels GeneticRNA Ribosomal 5SRNA Fungalbiology.organism_classificationRNA BacterialHorizontal gene transferOxidoreductasesSequence Alignment

description

A phylogenetic study of the isopenicillin-N-synthetase (IPNS) gene sequence from prokaryotic and lower eukaryotic producers of beta-lactam antibiotics by means of a maximum-likelihood approach has been carried out. After performing an extensive search, rather than invoking a global molecular clock, the results obtained are best explained by a model with three rates of evolution. Grouped in decreasing order, these correspond to A. nidulans and then to the rest of the eukaryotes and prokaryotes, respectively. The estimated branching date between prokaryotic and fungal IPNS sequences (852 +/- 106 MY) strongly supports the hypothesis that the IPNS gene was horizontally transferred from bacterial beta-lactam producers to filamentous fungi.

yearjournalcountryeditionlanguage
1996-05-01Journal of molecular evolution
10.1007/bf02352283https://pubmed.ncbi.nlm.nih.gov/8662005