6533b82ffe1ef96bd1295771

RESEARCH PRODUCT

Development of multidimensional spectroscopic and microscopic techniques to facilitate the monitoring of native fluorescence of biomolecules.

subject

description

There is a need for the development of rapid and reliable characterization tools for biological media. The objective of this thesis is to develop a method based on the acquisition of excitation-emission matrices of fluorescence (EEMF) coupled with the use of fluorescence lifetime measurement in spectroscopy and microscopy (FLIM). These techniques have great potential due to their speed, low sample volume required for analysis, non-destructive sample analysis, and low cost. This project focused on two biological media of great interest to the food industry: wine and bacterial spores. On one hand, we have a beverage representing a large world market, and on the other hand, a food contaminant responsible for various diseases. The coupling of our different techniques allowed us to characterize fluorescent probes native to these two biological environments and to evaluate their changes according to different parameters, such as malolactic fermentation or changes in pH and water activity. This tool focused on different native probes, with organic and phenolic acids in our wine matrix, and dipicolinic acid for the bacterial spore matrix. This project has allowed the construction of a database allowing the in-situ monitoring of these fluorescent probes in their native environment and gives rise to the implementation of a new method of characterization of biological environments.