6533b82ffe1ef96bd1295bcc
RESEARCH PRODUCT
Selection for reproduction under short photoperiods changes diapause-associated traits and induces widespread genomic divergence.
Johanna KinnunenAnna Lotta HiillosHannele KauranenAnneli HoikkalaR. Axel W. WibergPekka LankinenMichael G. RitchieDavid Hopkinssubject
0106 biological sciencesPhysiology030310 physiologyQH301 BiologyCircadian clockGenome Insect01 natural sciencestalvehtiminenkylmänkestävyyscircadian clockmedia_commonvuorokausirytmi0303 health sciencesluonnonvalintagenome analysesReproductionPhenotypeAdaptation PhysiologicalCircadian RhythmCold TemperatureDrosophila melanogasterPhenotypeFemaleReproductionLocomotionendocrine systemmahlakärpäsetPeriod (gene)media_common.quotation_subjectPhotoperiodZoologyreproductive diapausefotobiologiaAquatic ScienceDiapauseBiology010603 evolutionary biology03 medical and health sciencesQH301Quantitative Trait Heritablephotoperiodic timerAnimalsCircadian rhythmMolecular BiologyGeneEcology Evolution Behavior and SystematicsSelection (genetic algorithm)Critical day lenghtGenetic VariationDAScold tolerancelisääntyminenDiapauseChromosomes Insectcritical day lengthInsect ScienceperimähyönteisetLinear ModelsAnimal Science and Zoologydescription
The work has been supported by the Academyof Finland to A.H. (project 267244) and Natural Environment Research Council (NERC) funding (NE/J020818/1 to M.G.R.; NE/L501852/1 to R.A.W.W.). The incidence of reproductive diapause is a critical aspect of life history in overwintering insects from temperate regions. Much has been learned about the timing, physiology and genetics of diapause in a range of insects, but how the multiple changes involved in this and other photoperiodically regulated traits are inter-related is not well understood. We performed quasinatural selection on reproduction under short photoperiods in a northern fly species, Drosophila montana, to trace the effects of photoperiodic selection on traits regulated by the photoperiodic timer and/or by a circadian clock system. Selection changed several traits associated with reproductive diapause, including the critical day length for diapause (CDL), the frequency of diapausing females under photoperiods that deviate from daily 24 h cycles and cold tolerance, towards the phenotypes typical of lower latitudes. However, selection had no effect on the period of free-running locomotor activity rhythm regulated by the circadian clock in fly brain. At a genomic level, selection induced extensive divergence from the control line in 16 gene clusters involved in signal transduction, membrane properties, immunologlobulins and development. These changes resembled those detected between latitudinally divergent D. montana populations in the wild and involved SNP divergence associated with several genes linked with diapause induction. Overall, our study shows that photoperiodic selection for reproduction under short photoperiods affects diapause-associated traits without disrupting the central clock network generating circadian rhythms in fly locomotor activity. Publisher PDF Peer reviewed
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2019-01-01 | The Journal of experimental biology |