6533b82ffe1ef96bd12963f0

RESEARCH PRODUCT

Morphogenesis in callus and single-cell cultures ofLavandula latifoliaMedicus

subject

description

Procedures and factors promoting plant regeneration from callus and isolated cells were investigated in Lavandula latifolia Medicus. Callus was obtained from cotyledon explants cultured on MS medium supplemented with 0.34 (xM 2,4-D. These calli regenerated adventitious buds when transferred to MS medium containing either BA or several auxin (IAA or NAA)/BA combinations, best results being obtained in the presence of 8.80 (mi BA alone or in combination with 0.06 and 0.60 (xM IAA or NAA. Single cells from cotyledon-derived calli were able to proliferate as callus when plated in MS medium supplemented with IAA or several IAA/BA combinations. Best plating efficiency was achieved when cells were cultured at 1.5 x 104 cells/ml in the presence of 5.70 |iM IAA and 8.80 |iM BA. To induce organogenesis, cell-derived calli were transferred to several regeneration media. Maximum shoot differentiation occurred when cell-derived calli, grown in the presence of 8.80 ^M BA alone or in combination with 15% coconut milk. S...