0000000000754715
AUTHOR
Ana Maria Jordan
Morphogenesis in callus and single-cell cultures ofLavandula latifoliaMedicus
Procedures and factors promoting plant regeneration from callus and isolated cells were investigated in Lavandula latifolia Medicus. Callus was obtained from cotyledon explants cultured on MS medium supplemented with 0.34 (xM 2,4-D. These calli regenerated adventitious buds when transferred to MS medium containing either BA or several auxin (IAA or NAA)/BA combinations, best results being obtained in the presence of 8.80 (mi BA alone or in combination with 0.06 and 0.60 (xM IAA or NAA. Single cells from cotyledon-derived calli were able to proliferate as callus when plated in MS medium supplemented with IAA or several IAA/BA combinations. Best plating efficiency was achieved when cells were…
Plant regeneration from isolated cells ofLavandula latifolia medicus
Single cells were obtained from hypocotyl-derived callus ofLavandula latifolia Medicus. Cells were plated in Murashige and Skoog medium supplemented with indoleacetic acid (IAA), benzyladenine (BA), and several IAA-BA combinations. Cell division required the simultaneous presence of IAA and BA in the culture medium, but callus formation was only achieved with 0.1 or 1 mg/liter IAA and 2 mg/liter BA. To induce organogenesis, calli were transferred to various regeneration media. Shoot-bud differentiation efficiency depended on the composition of both the callus induction and the shoot regeneration media, best results being obtained when calli grown in 1 mg/liter IAA and 2 mg/liter BA were sub…
Micropropagation of adultLavandula dentataplants
SummaryA protocol for in vitro propagation of adult Lavandula dentata plants has been achieved. Cultures were established by placing nodal segments on Murashige and Skoog medium containing BA, KIN, and NAA. Highest shoot multiplication rates were obtained when explants grown in the presence of 5.0 μM BA or 20 (JLM KIN were transferred to medium with 8.8 μM BA and 15% coconut milk. Multiplication efficiency through subcultures was significantly affected by the cytokinin concentration in the initial culture medium. Subculture reduced drastically the final number of shoots produced on nodal segments isolated from shoots grown in the presence of 2.0 μM BA or 40.0 μM KIN. Shoots were easily root…