6533b870fe1ef96bd12d0436
RESEARCH PRODUCT
Micropropagation of adultLavandula dentataplants
Juan SeguraM. C. CalvoAna Maria Jordansubject
biologyfungifood and beveragesHorticulturebiology.organism_classificationLavandula dentatachemistry.chemical_compoundMurashige and Skoog mediumMicropropagationchemistryShootBotanyCytokininGeneticsKinetinSubculture (biology)Explant culturedescription
SummaryA protocol for in vitro propagation of adult Lavandula dentata plants has been achieved. Cultures were established by placing nodal segments on Murashige and Skoog medium containing BA, KIN, and NAA. Highest shoot multiplication rates were obtained when explants grown in the presence of 5.0 μM BA or 20 (JLM KIN were transferred to medium with 8.8 μM BA and 15% coconut milk. Multiplication efficiency through subcultures was significantly affected by the cytokinin concentration in the initial culture medium. Subculture reduced drastically the final number of shoots produced on nodal segments isolated from shoots grown in the presence of 2.0 μM BA or 40.0 μM KIN. Shoots were easily rooted on Murashige and Skoog hormone-free medium with macronutrients at half-strength. Plants were successfully transplanted into soil.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1998-01-01 | The Journal of Horticultural Science and Biotechnology |