6533b830fe1ef96bd1296e57
RESEARCH PRODUCT
Plant regeneration from isolated cells ofLavandula latifolia medicus
M. C. CalvoAna Maria JordanJuan Segurasubject
Callus formationClinical BiochemistryLavandula latifoliaOrganogenesisPlant ScienceCell BiologyBiologybiology.organism_classificationMurashige and Skoog mediumCell cultureCallusShootBotanyComposition (visual arts)BiotechnologyDevelopmental Biologydescription
Single cells were obtained from hypocotyl-derived callus ofLavandula latifolia Medicus. Cells were plated in Murashige and Skoog medium supplemented with indoleacetic acid (IAA), benzyladenine (BA), and several IAA-BA combinations. Cell division required the simultaneous presence of IAA and BA in the culture medium, but callus formation was only achieved with 0.1 or 1 mg/liter IAA and 2 mg/liter BA. To induce organogenesis, calli were transferred to various regeneration media. Shoot-bud differentiation efficiency depended on the composition of both the callus induction and the shoot regeneration media, best results being obtained when calli grown in 1 mg/liter IAA and 2 mg/liter BA were subcultured to media containing 2 mg/liter BA and 15% coconut milk. Under these conditions, up to 75% of calli formed shoots that subsequently were rooted and established in soil.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1988-09-01 | In Vitro Cellular & Developmental Biology |