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RESEARCH PRODUCT
Sperm kinematic, head morphometric and kinetic-morphometric subpopulations in the blue fox (Alopex lagopus)
Jesús ContellJan SegarvallLorena BoriMaría SanchoAnthony ValverdeCarles SolerAlmudena García-molinasubject
Maleendocrine systemUrologyPopulationFoxesMotilitySemenInvited Original ArticleKinematicsBiologyintegration of motility and morphologylcsh:RC870-923Sperm Preservationsperm morphometry03 medical and health sciences0302 clinical medicineAnimalseducationCell ShapesubpopulationSperm motilityPrincipal Component Analysiseducation.field_of_study030219 obstetrics & reproductive medicine0402 animal and dairy scienceintegration of motility and morphology; principal component analysis; sperm morphometry; subpopulation04 agricultural and veterinary sciencesGeneral MedicineAnatomylcsh:Diseases of the genitourinary system. UrologySpermatozoa040201 dairy & animal scienceSpermBiomechanical PhenomenaPrincipal component analysisSperm MotilitySperm Headdescription
This work provides information on the blue fox ejaculated sperm quality needed for seminal dose calculations. Twenty semen samples, obtained by masturbation, were analyzed for kinematic and morphometric parameters by using CASA-Mot and CASA-Morph system and principal component (PC) analysis. For motility, eight kinematic parameters were evaluated, which were reduced to PC1, related to linear variables, and PC2, related to oscillatory movement. The whole population was divided into three independent subpopulations: SP1, fast cells with linear movement; SP2, slow cells and nonoscillatory motility; and SP3, medium speed cells and oscillatory movement. In almost all cases, the subpopulation distribution by animal was significantly different. Head morphology analysis generated four size and four shape parameters, which were reduced to PC1, related to size, and PC2, related to shape of the cells. Three morphometric subpopulations existed: SP1: large oval cells; SP2: medium size elongated cells; and SP3: small and short cells. The subpopulation distribution differed between animals. Combining the kinematic and morphometric datasets produced PC1, related to morphometric parameters, and PC2, related to kinematics, which generated four sperm subpopulations - SP1: high oscillatory motility, large and short heads; SP2: medium velocity with small and short heads; SP3: slow motion small and elongated cells; and SP4: high linear speed and large elongated cells. Subpopulation distribution was different in all animals. The establishment of sperm subpopulations from kinematic, morphometric, and combined variables not only improves the well-defined fox semen characteristics and offers a good conceptual basis for fertility and sperm preservation techniques in this species, but also opens the door to use this approach in other species, included humans.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2016-09-01 | Asian Journal of Andrology |