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RESEARCH PRODUCT
Stable Expression of Heterologous Sulfotransferase in V79 Cells: Activation of Primary and Secondary Benzylic Alcohols
Andreas CzichJan PudilHansruedi GlattAlbrecht Seidelsubject
chemistry.chemical_classificationSulfotransferasePolymers and PlasticsbiologyOrganic ChemistryHeterologousGene mutationbiology.organism_classificationChinese hamsterchemistry.chemical_compoundEnzymeBiochemistrychemistryComplementary DNAMaterials ChemistryHydroxysteroidCytotoxicitydescription
Abstract A sulfotransferase (ST) capable of activating 1-hydroxymethylpyrene (HMP) and 9-hydroxymethylanthracene (HMA) to mutagens was purified from rat liver. This enzyme appeared to be identical with hydroxysteroid STa, whose cDNA was cloned and stably expressed in Chinese hamster V79 cells. Several primary and secondary benzylic alcohols derived from polycyclic aromatic hydrocarbons induced gene mutations, sister chromatid exchanges (SCE) and/or cytotoxicity in these cells.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1994-08-01 | Polycyclic Aromatic Compounds |