6533b830fe1ef96bd1297223
RESEARCH PRODUCT
MHC-unrestricted recognition of bacteria-infected target cells by human CD8+ cytotoxic T lymphocytes.
Werner-j. MayetElisabeth HermannKarl-hermann Meyer Zum BüschenfeldeBernhard Fleischersubject
AdultCytotoxicity ImmunologicMaleYersinia InfectionsCD3CD8 AntigensReceptors Antigen T-Cell alpha-betaImmunologyClone (cell biology)Human leukocyte antigenIn Vitro TechniquesMajor histocompatibility complexMicrobiologyCell LineMajor Histocompatibility ComplexT-Lymphocyte SubsetsCytotoxic T cellHumansYersinia enterocoliticaCells CulturedYersinia enterocoliticaImmunity CellularbiologyArthritisbiology.organism_classificationEnterobacteriaceaebiology.proteinCD8T-Lymphocytes Cytotoxicdescription
Abstract A CD8 + αβTCR + T cell clone (A35) was isolated from the synovial fluid of a patient with postenteric reactive arthritis caused by Yersinia enterocolitica . This clone efficiently killed autologous and allogeneic target cells that had been preincubated with live but not with heat-killed bacteria. There was no restriction by polymorphic parts of HLA-A, -B. or -C molecules and a HLA class II-deficient mutant cell line was lysed as efficiently as its normal counterpart, whereas infected HLA class I-deficient cells (Daudi cells) were not. The clone showed crossreaction between Yersinia enterocolitica , Escherichia coli , Pseudomonas aeruginosa , and Streptococcus pyogenes , but did not lyse target cells preincubated with Staphylococcus epidermidis . MAb to CD2. CD3. and CD8 efficiently blocked A35. whereas the addition of mAb to HLA class II or to HLA class I did not. This clone apparently represents a novel effector mechanism against bacteria-infected or -modified cells that could be involved in the immunopathology of reactive arthritis.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1992-09-01 | Cellular immunology |