6533b830fe1ef96bd129725a

RESEARCH PRODUCT

Anaphylatoxin-like molecules generated during complement activation induce a dramatic enhancement of particle uptake in rainbow trout phagocytes.

Jun LiS LapatraR. PetersJ.o. SunyerMirella Vazzana

subject

AnaphylatoxinsTime FactorsEvolutionPhagocytosisImmunologySize-exclusion chromatographyComplementAnaphylatoxinPhagocytosisCell MovementLeukocytesAnimalsAnaphylatoxinPhagocytesPhagocytosibiologyChemotaxisC4AChemotaxisComplement System Proteinsbiology.organism_classificationFlow CytometryMolecular biologyComplement systemTroutRainbow troutOncorhynchus mykissImmunologybiology.proteinAntibodyDevelopmental Biology

description

Here we have identified a serum fraction containing approximately 8-kDa molecules with an unexpected capacity to greatly enhance particle uptake in trout head kidney leukocytes (HKLs). This approximately 8-kDa particle-uptake enhancing fraction (PUEF-8) was purified from complement-activated serum by gel filtration chromatography. Mass spectrometric analysis and reactivity of anti-trout C3-1 and C4 antibodies, indicated the presence of C3a, C4a and C5a molecules in PUEF-8. Using a newly developed flow cytometric assay that measures the capacity of cells to ingest fluorescent beads, we showed that PUEF-8 induced a striking enhancement (344+/-50% higher than the PBS control value) in the number of HKLs ingesting three or more beads. In contrast, the effect of PUEF-8 on peripheral blood leukocytes (PBLs) was almost negligible. Interestingly, PUEF-8 acted as a strong chemoattractant for both HKLs and PBLs. These findings suggest a novel role for the anaphylatoxins generated during complement activation in teleost fish.

yearjournalcountryeditionlanguage
2004-08-01
10.1016/j.dci.2004.03.004http://hdl.handle.net/10447/11474