Search results for "Complement"
showing 10 items of 2113 documents
On two classes of finite supersoluble groups
2017
ABSTRACTLet ℨ be a complete set of Sylow subgroups of a finite group G, that is, a set composed of a Sylow p-subgroup of G for each p dividing the order of G. A subgroup H of G is called ℨ-S-semipermutable if H permutes with every Sylow p-subgroup of G in ℨ for all p∉π(H); H is said to be ℨ-S-seminormal if it is normalized by every Sylow p-subgroup of G in ℨ for all p∉π(H). The main aim of this paper is to characterize the ℨ-MS-groups, or groups G in which the maximal subgroups of every Sylow subgroup in ℨ are ℨ-S-semipermutable in G and the ℨ-MSN-groups, or groups in which the maximal subgroups of every Sylow subgroup in ℨ are ℨ-S-seminormal in G.
Electrostatic complementarity in pseudoreceptor modeling based on drug molecule crystal structures: the case of loxistatin acid (E64c)
2015
After a long history of use as a prototype cysteine protease inhibitor, the crystal structure of loxistatin acid (E64c) is finally determined experimentally using intense synchrotron radiation, providing insight into how the inherent electronic nature of this protease inhibitor molecule determines its biochemical activity. Based on the striking similarity of its intermolecular interactions with those observed in a biological environment, the electrostatic potential of crystalline E64c is used to map the characteristics of a pseudo-enzyme pocket.
Highly efficient construction of infectious viroid-derived clones
2019
[Background] Viroid research generally relies on infectious cDNA clones that consist of dimers of the entire viroid sequence. At present, those dimers are generated by self-ligation of monomeric cDNA, a strategy that presents several disadvantages: (i) low efficiency, (ii) it is a non-oriented reaction requiring tedious screenings and (iii) additional steps are required for cloning into a binary vector for agroinfiltration or for in vitro RNA production.
Environmental DNA effectively captures functional diversity of coastal fish communities.
2020
Robust assessments of taxonomic and functional diversity are essential components of research programmes aimed at understanding current biodiversity patterns and forecasting trajectories of ecological changes. Yet, evaluating marine biodiversity along its dimensions is challenging and dependent on the power and accuracy of the available data collection methods. Here we combine three traditional survey methodologies (underwater visual census strip transects [UVCt], baited underwater videos [BUV] and small-scale fishery catches [SSFc]), and one novel molecular technique (environmental DNA metabarcoding [eDNA]-12S rRNA and cytochrome oxidase subunit 1 [COI]) to investigate their efficiency and…
Characterization of the Copper Transporters from Lotus spp. and Their Involvement under Flooding Conditions
2019
Forage legumes are an important livestock nutritional resource, which includes essential metals, such as copper. Particularly, the high prevalence of hypocuprosis causes important economic losses to Argentinian cattle agrosystems. Copper deficiency in cattle is partially due to its low content in forage produced by natural grassland, and is exacerbated by flooding conditions. Previous results indicated that incorporation of Lotus spp. into natural grassland increases forage nutritional quality, including higher copper levels. However, the biological processes and molecular mechanisms involved in copper uptake by Lotus spp. remain poorly understood. Here, we identify four genes that encode p…
Responsiveness of metallothionein and hemocyanin genes to cadmium and copper exposure in the garden snail Cornu aspersum.
2020
Abstract Terrestrial gastropods express metal‐selective metallothioneins (MTs) by which they handle metal ions such as Zn2+, Cd2+, and Cu+/Cu2+ through separate metabolic pathways. At the same time, they depend on the availability of sufficient amounts of Cu as an essential constituent of their respiratory protein, hemocyanin (Hc). It was, therefore, suggested that in snails Cu‐dependent MT and Hc pathways might be metabolically connected. In fact, the Cu‐specific snail MT (CuMT) is exclusively expressed in rhogocytes, a particular molluscan cell type present in the hemocoel and connective tissues. Snail rhogocytes are also the sites of Hc synthesis. In the present study, possible interacti…
cDNA sequences of two arylphorin subunits of an insect biliprotein: phylogenetic differences and gene duplications during evolution of hexamerins-imp…
2016
Arylphorins represent a conserved class of hexameric ∼500 kDa insect hemolymph glycoproteins, rich in aromatic amino acids, which are produced in large quantities at the larval stage as reserves for metamorphosis and egg development. The recently isolated arylphorin from the moth Cerura vinula is unique in being complexed to a novel farnesylated bilin. Protein sequencing suggested the presence of two different ∼85 kDa subunits. Here, we report the complete coding sequences of two cDNAs encoding two arylphorins subunits with 67% identity and calculated physicochemical characteristics in agreement with the isolated holoprotein. Our phylogenetic analyses of the hexamerins revealed monophyletic…
Extraction and Analysis of Carotenoids from Escherichia coli in Color Complementation Assays
2017
A common method to investigate the function of genes putatively involved in carotenoid biosynthesis is the so called color complementation assay in Escherichia coli (see, e.g., Cunningham and Gantt, 2007). In this assay, the gene under investigation is expressed in E. coli strains genetically engineered to synthesize potential carotenoid substrates, followed by analysis of the pigment changes in the carotenogenic bacteria via high-performance liquid chromatography (HPLC). Two crucial steps in this method are (i) the quantitative extraction of the carotenoids out of E. coli and (ii) the reproducible and complete separation of the pigments by HPLC. Here, we present a protocol for the extracti…
Evolutionary diversification of type-2 HDAC structure, function and regulation in Nicotiana tabacum
2018
Ministère de l'Education Nationale et de la Recherche ; Conseil Régional de Bourgogne (PARI AGRALE8) ; Association pour la Recherche sur les Nicotianacées ; Conseil Régional de Bourgogne; International audience; Type-2 HDACs (HD2s) are plant-specific histone deacetylases that play diverse roles during development and in responses to biotic and abiotic stresses. In this study we characterized the six tobacco genes encoding HD2s that mainly differ by the presence or the absence of a typical zinc finger in their C-terminal part. Of particular interest, these HD2 genes exhibit a highly conserved intron/exon structure. We then further investigated the phylogenetic relationships among the HD2 gen…
Essential Oil Yield, Chemical Composition, and Total Citral Content of Nine Cultivars of Cymbopogon Species from Western India
2016
ABSTRACTThe essential oil composition of five elite Cymbopogon varieties (OD-19, Pragati, RRL-16, NLG-84) and a hybrid (CPK-25) and four new selections (LS-1 to LS-4) were determined by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The oil yield ranged from 0.31% to 1.0% with CPK-25 (1.0%) and LS-2 (0.95%) containing the highest. A total of 66 compounds, constituting 84.6–94.3% of the oils, were identified. The main compounds were geranial (29.9–41.6%), neral (25.8–32.5%), citronellol (0.3–12.8%), geranyl acetate (1.2–8.4%), and geraniol (1.8–8.2%). Of the cultivars, OD-19 (74.1%) had the highest and LS-3 (55.7%) contained the lowest amount of total citral. CKP-2…