Highly efficient construction of infectious viroid-derived clones

6533b7ddfe1ef96bd1274858

RESEARCH PRODUCT

Highly efficient construction of infectious viroid-derived clones

Vicente Pallás Gustavo Gómez José Antonio Navarro Joan Marquez-molins

subject

0106 biological sciences 0301 basic medicine Agroinfiltration IIs enzymes Viroid viruses Plant Science Computational biology lcsh:Plant culture Biology 01 natural sciences 03 medical and health sciences Agro-infiltration Complementary DNA Genetics Lethal allele lcsh:SB1-1110 Vector (molecular biology)Dimers lcsh:QH301-705.5 Cloning Viroid Methodology RNA biology.organism_classification Restriction enzyme 030104 developmental biology lcsh:Biology (General)010606 plant biology & botany Biotechnology Cloning

description

[Background] Viroid research generally relies on infectious cDNA clones that consist of dimers of the entire viroid sequence. At present, those dimers are generated by self-ligation of monomeric cDNA, a strategy that presents several disadvantages: (i) low efficiency, (ii) it is a non-oriented reaction requiring tedious screenings and (iii) additional steps are required for cloning into a binary vector for agroinfiltration or for in vitro RNA production.

year	journal	country	edition	language
2019-08-01

10.1186/s13007-019-0470-4 https://hdl.handle.net/10251/160075

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