Search results for "Agro-infiltration"

showing 2 items of 2 documents

Highly efficient construction of infectious viroid-derived clones

2019

[Background] Viroid research generally relies on infectious cDNA clones that consist of dimers of the entire viroid sequence. At present, those dimers are generated by self-ligation of monomeric cDNA, a strategy that presents several disadvantages: (i) low efficiency, (ii) it is a non-oriented reaction requiring tedious screenings and (iii) additional steps are required for cloning into a binary vector for agroinfiltration or for in vitro RNA production.

0106 biological sciences0301 basic medicineAgroinfiltrationIIs enzymesViroidvirusesPlant ScienceComputational biologylcsh:Plant cultureBiology01 natural sciences03 medical and health sciencesAgro-infiltrationComplementary DNAGeneticsLethal allelelcsh:SB1-1110Vector (molecular biology)Dimerslcsh:QH301-705.5CloningViroidMethodologyRNAbiology.organism_classificationRestriction enzyme030104 developmental biologylcsh:Biology (General)010606 plant biology & botanyBiotechnologyCloning

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Symptom severity, infection progression and plant responses in solanum plants caused by three pospiviroids vary with the inoculation procedure

2021

This article belongs to the Section Molecular Plant Sciences.

0106 biological sciences0301 basic medicineViroidvirusesPospiviroidaeCEVd01 natural sciencesRibosome18S ribosomal RNAAgro-infiltrationSolanum lycopersicumBiology (General)Spectroscopyfood and beveragesGeneral MedicinePSTVdTranscriptRibosomeViroidsComputer Science Applications02.- Poner fin al hambre conseguir la seguridad alimentaria y una mejor nutrición y promover la agricultura sostenibleChemistryRNA ViralPlasmidsQH301-705.5PospiviroidaeBiologyEggplantStressArticleCatalysisTomatoMicrobiologyInorganic Chemistry03 medical and health sciencesBIOQUIMICA Y BIOLOGIA MOLECULARPhysical and Theoretical ChemistryQD1-999Molecular BiologyPotato spindle tuber viroidPlant DiseasesInoculationOrganic ChemistryfungiRibosomal RNAbiology.organism_classification030104 developmental biologyTCDVdSolanumRibosomes010606 plant biology & botany

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