6533b830fe1ef96bd12979a5
RESEARCH PRODUCT
Changes in collagen cross-linking of articular cartilage are revealed by spectral reflectance imaging
Vuokko KovanenJussi KinnunenHarri T. KokkonenJukka S. JurvelinMarkku Hauta-kasariPasi Vahimaasubject
chemistry.chemical_compoundCollagen cross linkingThreoseChemistryIn vivoArticular cartilageReflectivityOptical reflectionBiomedical engineeringdescription
Excessive cross-linking of collagen during aging may contribute to degeneration of articular cartilage. Traditionally, the amount of cross-links is derived by using destructive high-performance liquid chromatography (HPLC). However, a sensitive, non-destructive method could help to evaluate tissue integrity, including cross-links. We increased collagen cross-linking in bovine articular cartilage by controlled threose incubation. During the incubation, optical spectral reflectance images of the samples were captured and related to HPLC results using regression analysis. Significant correlations with reflectance and cross-links were observed. When further developed the optical method may enable nondestructive evaluation of cross-links in vivo through a clinical arthroscope.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2012-06-01 | 2012 25th IEEE International Symposium on Computer-Based Medical Systems (CBMS) |