6533b830fe1ef96bd1297a5d
RESEARCH PRODUCT
Mapping candidate genes for Drosophila melanogaster resistance to the parasitoid wasp Leptopilina boulardi.
M. HitaL. PascualEric EspagneFrançoise LemeunierMarylène PoiriéGeorges PeriquetYves Cartonsubject
Male0106 biological sciencesCandidate geneWaspsGenes Insect010603 evolutionary biology01 natural sciencesParasitoid wasp03 medical and health sciencesGenes RegulatorGeneticsAnimalsDrosophila Proteins[SDV.BV]Life Sciences [q-bio]/Vegetal BiologyAlleleGeneIn Situ Hybridization030304 developmental biologyRecombination GeneticGenetics0303 health sciences[SDV.GEN]Life Sciences [q-bio]/GeneticsModels GeneticbiologyBreakpointIntracellular Signaling Peptides and ProteinsChromosome MappingMembrane ProteinsChromosomeGeneral MedicineCosmidsbiology.organism_classificationDrosophila melanogasterLarvaDrosophila melanogasterRecombinationdescription
Drosophila melanogaster resistance against the parasitoid wasp Leptopilina boulardi is under the control of a single gene (Rlb), with two alleles, the resistant one being dominant. Using strains bearing deletions, we previously demonstrated that the 55E2–E6; 55F3 region on chromosome 2R is involved in the resistance phenomenon. In this paper, we first restricted the Rlb containing region by mapping at the molecular level the breakpoints of the Df(2R)Pc66, Df(2R)P34 and Df(2R)Pc4 deficiencies, using both chromosomal in situ hybridization and Southern analyses. The resistance gene was localized in a 100 kb fragment, predicted to contain about 10 different genes. Male recombination genetic experiments were then performed, leading to identification of two possible candidates for the Rlb gene. Potential involvement of one of this genes, edl/mae, is discussed.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2006-11-13 |