6533b830fe1ef96bd1297b08
RESEARCH PRODUCT
Expression of Staphylococcus saprophyticus surface properties is modulated by composition of the atmosphere.
Heinz-georg W. MeyerSören G. Gatermannsubject
Microbiology (medical)food.ingredientStaphylococcusImmunologyBiologyMicrobiologychemistry.chemical_compoundfoodBacterial ProteinsImmunology and AllergyAgarAdhesins Bacterialchemistry.chemical_classificationGrowth mediumStaphylococcus saprophyticusOsmotic concentrationGeneral MedicineHemagglutininbiology.organism_classificationFibronectinsMolecular WeightHemagglutininsFibronectin bindingchemistryBiochemistryGlycoproteinCarrier ProteinsBacteriaBacterial Outer Membrane Proteinsdescription
Expression of two major surface proteins of Staphylococcus saprophyticus, the haemagglutinin and the Staphylococcus saprophyticus surface-associated protein (Ssp), requires carefully defined culture conditions. The Ssp is produced when bacteria are grown on agar, whereas expression of the haemagglutinin requires growth in broth. We sought to identify the environmental signals that are responsible for this modulation. Varying the pH, the osmolarity of the growth medium or the temperature did not influence expression of the proteins. In contrast, growth in an anaerobic atmosphere increased haemagglutination titres and fibronectin binding (both mediated by the haemagglutinin) but suppressed production of the Ssp. As the influence of the CO2 level could be excluded, we conclude that expression of these surface proteins is probably modulated by the O2 content of the atmosphere.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1995-08-01 | Medical microbiology and immunology |