6533b830fe1ef96bd1297bfe

RESEARCH PRODUCT

Inhibition of neuronally mediated secretion in rat colonic mucosa by prostaglandin D2

Walter RummelR. WanitschkeKarl J. GoergMartin Diener

subject

Serotoninmedicine.medical_specialtyColonProstaglandinSecretomotorSubstance PSubstance PBiologyDinoprostMembrane Potentialschemistry.chemical_compoundBody WaterChloridesInternal medicinemedicineAnimalsIloprostIntestinal MucosaProstaglandin E2NeurotensinHepatologyUssing chamberProstaglandin D2SodiumGastroenterologyRats Inbred Strainsrespiratory systemEpoprostenolRatsEndocrinologychemistryDiffusion Chambers CultureFemalelipids (amino acids peptides and proteins)SerotoninProstaglandin D2Neurotensinmedicine.drug

description

Abstract The effect of prostaglandin D 2 (PGD 2 ) on ion transport across the mucosa of the descending colon was studied in rats. PGD 2 dose-dependently decreased baseline short-circuit current of mucosa-submucosal preparations mounted either in the Ussing chamber or mounted as an everted sac. However, with the everted sac technique, the tissue was about 1000 times more sensitive to PGD 2 . Concomitant with the decrease in short-circuit current, PGD 2 increased the mucosal-to-serosal fluxes of sodium and chloride and decreased the serosal-tomucosal flux of chloride. PGD 2 inhibited the secretory action of the PGI 2 analogue iloprost, PGD 2α , and neurotensin. The action of these secretagogues was dependent on the presence of the submucosal plexus. In contrast, PGD 2 had no effect on the increase in short-circuit current caused by PGD 2 , substance P, or serotonin, the actions of which were not dependent on the presence of the submucosal plexus. The results indicate that the action site of the antisecretory mechanism of PGD 2 is localized in the secretomotor neurons.

yearjournalcountryeditionlanguage
1992-09-01Gastroenterology
https://doi.org/10.1016/0016-5085(92)90006-k