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RESEARCH PRODUCT

Variable zoom digital in-line holographic microscopy

Maciej TrusiakVicente MicóJavier GarciaMartín Sanz

subject

Physicsbusiness.industryMechanical EngineeringResolution (electron density)HolographyRanging02 engineering and technology021001 nanoscience & nanotechnology01 natural sciencesAtomic and Molecular Physics and OpticsElectronic Optical and Magnetic Materialslaw.invention010309 opticsLens (optics)Opticslaw0103 physical sciencesMicroscopyDigital image processingElectrical and Electronic EngineeringZoom0210 nano-technologyPhase retrievalbusiness

description

Abstract We report on a novel layout providing variable zoom in digital in-line holographic microscopy (VZ-DIHM). The implementation is in virtue of an electrically tunable lens (ETL) which enables to slightly shift the illumination source axial position without mechanical movement of any system component. Magnifications ranging from ~15X to ~35X are easily achievable using the same layout and resulting in a substantial variation of the total field of view (FOV). The performance of the proposed setup is, first, validated using a resolution test target where the main parameters are analyzed (theoretically and experimentally) and, second, corroborated analyzing biological sample (prostate cancer cells) showing its application to biomedical imaging.

yearjournalcountryeditionlanguage
2020-04-01Optics and Lasers in Engineering
https://doi.org/10.1016/j.optlaseng.2019.105939