6533b831fe1ef96bd1298429

RESEARCH PRODUCT

Identification of Type I and IX Collagens in the Ascidian Ciona intestinalis

Vincenzo ArizzaEleonora PatricoloAiti VizziniNicolò ParrinelloRoberto GambinoMelchiorre CervelloMatteo CammarataCinzia Chinnici

subject

chemistry.chemical_classificationbiologychemistryComplementary DNACiona intestinalisNorthern blotbiology.organism_classificationRepeated sequenceMolecular biologyHomology (biology)Paracentrotus lividusType I collagenAmino acid

description

Immunohistochemical methods showed that a type I collagen is a component of the tunic of Ciona intestinalis, involved in the encapsulation process. Since the fibril-forming collagen types are characterized by triple helical domain with a highly preserved Gly-Xaa-Yaa repeated sequence, a probe coding the fibril-forming type I collagen of the echinoderm Paracentrotus lividus was used to identify ascidian cDNA clones. Northern blot hybridization established that P. lividus probe cross-hybridizes with a 6 Kb C. intestinalis mRNA isolated from the pharynx. Using the echinodermal type I collagen cDNA as a probe several positive clones were identified. Analysis of sequence and the deduced amino acid sequences of 2.5 Kb clone indicate a strong homology with the type IX collagen of human, rat and mouse.

yearjournalcountryeditionlanguage
2001-01-01
https://doi.org/10.1007/978-4-431-66982-1_59