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RESEARCH PRODUCT
Can MALDI-TOF Mass Spectrometry Reasonably Type Bacteria?
Xavier BertrandBenoît ValotMarlène SaugetDidier Hocquetsubject
0301 basic medicineMicrobiology (medical)Staphylococcus aureus030106 microbiologyStatistics as TopicComputational biologyBiologyMass spectrometryMicrobiologyMicrobiology03 medical and health sciencesSpecies Specificity[ SDV.MP ] Life Sciences [q-bio]/Microbiology and ParasitologyVirologyEscherichia coliSpecies identificationMALDI-TOF MSTypingBacteriaLimitingTypingbiology.organism_classificationMALDI-TOF Mass SpectrometryBacterial Typing TechniquesMatrix-assisted laser desorption/ionizationInfectious Diseases[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologySpectrometry Mass Matrix-Assisted Laser Desorption-IonizationTyping methodsBacteriaBiomarkersdescription
International audience; Bacterial typing is crucial to tackle the spread of bacterial pathogens but current methods are time-consuming and costly. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been recently integrated into the microbiology laboratory workflow for a quick and low-cost microbial species identification. Independent research groups have successfully redirected the original function of this technology from their primary purpose to discriminate subgroups within pathogen species. However, identical bacterial subgroups could be identified by unrelated peaks by independent methods, thus limiting their robustness and exportability. We propose several guidelines that could improve the performance of MALDI-TOF MS-based typing methods for use as a first-line epidemiological tool.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-01-13 |