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RESEARCH PRODUCT
High-Speed Video Microscopy for Primary Ciliary Dyskinesia Diagnosis: A Study of Ciliary Motility Variations with Time and Temperature
Javier Milara PayáJavier MilaraJavier Pitarch-fabregatMiguel ArmengotLara MilianManuel Mata-roigJulio CortijoAna Reulasubject
medicine.medical_specialtyMedicine (General)Kartagener's syndrome - Diagnosis.Respiratory organs - Diseases in children.Clinical BiochemistryAparato respiratorio - Enfermedades en los niños.Video microscopyArticlehigh-speed video microscopy (HSVM)03 medical and health sciences0302 clinical medicineR5-920ciliary beat frequency (CBF)Microscopia.OphthalmologyMicroscopy.MicroscopymedicinePediatric otolaryngology.030212 general & internal medicinePrimary ciliary dyskinesiaDisquinesia ciliar Síndrome de - Diagnóstico.primary ciliary dyskinesia (PCD)business.industryPrimary ciliary dyskinesia - Diagnosis.Enfermedades crónicas en los niños.Otorrinolaringología pediátrica.Gold standard (test)Ciliary motilitymedicine.diseaseNasal epitheliumChronic diseases in children.ciliary beat pattern (CBP)High speed video030228 respiratory systemKartagener Síndrome de - Diagnóstico.Sample collectionbusinessdescription
Este artículo se encuentra disponible en la siguiente URL: https://www.mdpi.com/2075-4418/11/7/1301 Este artículo de investigación pertenece al número especial "Progress in Diagnosing and Managing Primary Ciliary Dyskinesia". Primary ciliary dyskinesia (PCD) is a rare disease resulting from a defect in ciliary function that generates, among other issues, chronic upper and lower respiratory tract infections. European guidelines recommend studying ciliary function (pattern (CBP) and frequency (CBF)), together with characteristic clinical symptoms, as one of the definitive tests. However, there is no “gold standard”. The present study aims to use high-speed video microscopy to describe how CBF and CBP alter over time and at different temperatures to reduce the error rate in the diagnosis of PCD. Samples of nasal epithelium from 27 healthy volunteers were studied to assess CBF and CBP at 0, 3, 24, 48, and 72 h, at room temperature and 4 C. It was observed that CBF increased while CBP became dyskinetic, both at room temperature and at 4 C, as time passed, especially after 3 h. In order to preserve all ciliary function parameters and to perform a reliable analysis to improve the diagnostic process of PCD, analysis should be performed within the first 3 h of sample collection, preferably in reference centers.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2021-07-20 |