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RESEARCH PRODUCT
Citrate permease gene expression inLactococcus lactissubsp.lactisstrains IL1403 and MG1363
Gerald BourelCharles DivièsHervé PrévostSadjia Bekalsubject
biologyATP citrate lyasePermeaseAcetoinBiovarLactococcus lactisMutantfood and beveragesbiology.organism_classificationLyaseMicrobiologyDiacetylMicrobiologychemistry.chemical_compoundchemistryBiochemistryGeneticsMolecular Biologydescription
Citrate permease gene expression in the plasmid-free Lactococcus lactis strains IL1403 and MG1363 was studied. The ability to transport citrate results in diacetyl and acetoin production in IL1403 but not in MG1363. Citrate lyase, α-acetolactate decarboxylase, diacetyl and acetoin reductase were detected in IL1403. These data show that L. lactis ssp. lactis strain IL1403 is a citrate permease mutant of the biovar. diacetylactis. Immunological analysis revealed the α-and β-subunits of citrate lyase not only in IL1403 but also in MG1363 where no citrate lyase activity was found.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1996-12-01 | FEMS Microbiology Letters |