6533b831fe1ef96bd1299a18
RESEARCH PRODUCT
Acid sensitivity of neomycin-resistant mutants ofOenococcus oeni: a relationship between reduction of ATPase activity and lack of malolactic activity
Jean GuzzoCharles DivièsLouis-charles FortierRaphaëlle Tourdot-maréchalByong H. Leesubject
ATPaseMutantMalatesMicrobiologyMicrobiologyGeneticsmedicineMalolactic fermentationLactic AcidMolecular BiologyHeat-Shock ProteinsOenococcus oeniAdenosine Triphosphataseschemistry.chemical_classificationbiologyStrain (chemistry)Drug Resistance MicrobialNeomycinNeomycinHydrogen-Ion Concentrationbiology.organism_classificationAnti-Bacterial AgentsGram-Positive CocciEnzymeBiochemistrychemistrybiology.proteinHeat-Shock ResponseLeuconostocBacteriamedicine.drugdescription
Mutants of Oenococcus oeni were isolated as spontaneous neomycin-resistant mutants. Three of these mutants harbored a significantly reduced ATPase activity that represented 50% of that of the wild-type strain. Their growth rates were also impaired at pH 5.3 (46-86% of the wild-type level). However, the profiles of sugar consumption appeared identical to those of the parental strain. At pH 3.2, all the mutant strains failed to grow and a drastic decrease in viability was observed after an acid shock. Surprisingly, all the isolated mutants were devoid of malolactic activity. These results suggest that the ATPase and malolactic activities of O. oeni are linked to each other and play a crucial role in the mechanism of resistance to an acid stress.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1999-09-01 | FEMS Microbiology Letters |