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RESEARCH PRODUCT

Impact of a new bio-pesticide produced by Paenibacillus sp. strain B2 on the genetic structure and density of soil bacterial communities

Silvio GianinazziNadine RouardFabrice Martin-laurentDiederik Van TuinenSameh Selim

subject

[SDV]Life Sciences [q-bio]QUANTITATIVE POLYMERASE CHAIN REACTIONMicrobiologyBIOPESTICIDE03 medical and health sciencesPaenibacillusDenitrifying bacteriaDry weightRNA Ribosomal 16SPAENIMYXINDNA Ribosomal SpacerFood sciencePolymyxinsPesticidesSoil Microbiology030304 developmental biology0303 health sciencesbiologyStrain (chemistry)Bacteria030306 microbiologyGeneral MedicineSequence Analysis DNAbiology.organism_classification16S ribosomal RNADNA Fingerprinting3. Good healthBiopesticideMicrobial population biologySOIL DEOXYRIBONUCLEIC ACIDInsect Science[SDE]Environmental SciencesAgronomy and Crop ScienceBacteria

description

The effect of paenimyxin, a new biopesticide produced by Paenibacillus sp. strain B2, on the density of soil bacterial communities was assessed by colony counting and by 16S rDNA and nirK quantitative polymerase chain reaction (PCR). Paenimyxin had a negative effect on the bacterial colony-forming unit (CFU) number, which was significantly reduced 2 and 4 days after treatment. The effect of paenimyxin on cultivatable bacteria was negligible 7 days after treatment. Approximately 107 16S rDNA sequences per gram of soil (dry weight) were detected by quantitative PCR in all samples. Paenimyxin did not affect the quantification of 16S rDNA or of the denitrifying bacterial community. In addition, RISA fingerprinting showed that the genetic structure of the bacterial communities was significantly modified 2 days after paenimyxin application at 50 µM and 4 days after treatment at lower concentrations (0.5 and 5 µM). The impact of paenimyxin treatment on the genetic structure of soil bacterial communities was transient, as no effect could be observed after 7, 14 and 28 days when compared with the untreated control. Copyright © 2007 Society of Chemical Industry

yearjournalcountryeditionlanguage
2007-01-25
10.1002/ps.1335https://hal.inrae.fr/hal-02659937