6533b832fe1ef96bd129a29e
RESEARCH PRODUCT
FRET-based method for evaluation of the efficiency of reversible and irreversible sonoporation.
Paulius RuzgysSaulius ŠAtkauskasVanesa LukinsoneMindaugas Tamošiūnassubject
0301 basic medicineMaterials scienceCell SurvivalSonicationSingle measurementBiomedical EngineeringCHO CellsBiomaterials03 medical and health scienceschemistry.chemical_compoundSonicationCricetulusEthidiumFluorescence Resonance Energy TransferAnimalsHumansViability assayFluorescent DyesTrypan BlueFluorescenceAtomic and Molecular Physics and OpticsElectronic Optical and Magnetic Materials030104 developmental biologyFörster resonance energy transferchemistryBiophysicsTrypan blueEthidium bromideSonoporationdescription
It is widely known that not all of the treated cells survive after introduction of exogenous molecules via any physical method. Therefore, it is important to develop methods that would allow simultaneous evaluation of both molecular delivery efficiency and cell viability. This study presents Forster resonance energy transfer (FRET)-based method that allows molecular transfer and cell viability evaluation in a single measurement by employing two common fluorescent dyes, namely, ethidium bromide and trypan blue. The method has been validated using cell sonoporation. The FRET-based method allows the efficiency evaluation of both reversible and irreversible sonoporation in a single experiment. Therefore, this method could be used to reduce time, labor, and material cost while improving the accuracy of evaluations.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-09-14 | Journal of biomedical optics |