6533b832fe1ef96bd129a3a6
RESEARCH PRODUCT
In situ forming hydrogels of hyaluronic acid and inulin derivatives for cartilage regeneration.
Giovanna PitarresiGiovanna PitarresiAlessandro GulinoMauro Di StefanoFabio Salvatore PalumboStefano AgnelloGaetano GiammonaCalogero Fioricasubject
Polymers and PlasticsPolymersInulinmacromolecular substancesHydrolysischemistry.chemical_compoundChondrocytesTissue engineeringHyaluronidaseHyaluronic acidPolymer chemistryMaterials ChemistrymedicineAnimalsRegenerationHyaluronic Acidchemistry.chemical_classificationTissue EngineeringChemistryOrganic Chemistrytechnology industry and agricultureInulinHydrogelsPolymerhydrogels hyaluronic acid inulinCartilageCross-Linking ReagentsSettore CHIM/09 - Farmaceutico Tecnologico ApplicativoSelf-healing hydrogelsMichael reactionMicroscopy Electron ScanningCattlemedicine.drugdescription
An in situ forming hydrogel obtained by crosslinking of amino functionalized hyaluronic acid derivatives with divinylsulfone functionalized inulin (INU-DV) has been here designed and characterized. In particular two hyaluronic acid derivatives bearing respectively a pendant ethylenediamino (EDA) portion (HA-EDA) and both EDA and octadecyl pendant groups (HA-EDA-C18) were crosslinked through an azo-Michael reaction with INU-DV. Gelation time and consumption of DV portions have been evaluated on hydrogel obtained using HA-EDA and HA-EDA-C18 derivatives with a concentration of 3% w/v and a ratio 80/20 w/w respect to the crosslinker INU-DV. The presence of pendant C18 chains improves mechanical performances of hydrogels and decreases the susceptibility to hyaluronidase hydrolysis. Bovine chondrocytes, encapsulated during crosslinking, sufficiently survive and efficiently proliferate until 28 days of analysis.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2014-07-29 | Carbohydrate polymers |