6533b832fe1ef96bd129a40b
RESEARCH PRODUCT
Genome-wide association meta-analysis highlights light-induced signaling as a driver for refractive error
Ms TedjaR WojciechowskiPg HysiN ErikssonNa FurlotteVjm VerhoevenAi IglesiasMa Meester-smoorSw TompsonQ FanAp KhawajaC-y ChengR HöhnK YamashiroA WenocurC GrazalT HallerA MetspaluJ WedenojaJb JonasYx WangJ XieP MitchellPj FosterBek KleinR KleinAd PatersonSm HosseiniRl ShahC WilliamsYy TeoYc ThamP GuptaW ZhaoY ShiW-y SawE-s TaiXl SimJe HuffmanO PolašekC HaywardG BencicI RudanJf WilsonCream Consortium23andme Research TeamUk Biobank Eye And Vision ConsortiumPk JoshiA TsujikawaF MatsudaKn WhisenhuntT ZellerPj Van Der SpekR HaakH Meijers-heijboerEm Van LeeuwenSk IyengarJh LassA HofmanF RivadeneiraAg UitterlindenJr VingerlingT LehtimäkiOt RaitakariG BiinoMp ConcasT-h Schwantes-anRp IgoG Cuellar-partidaNg MartinJe CraigP GharahkhaniKm WilliamsA NagJs RahiPm CumberlandC DelcourtC BellenguezJs RiedAa BergenT MeitingerC GiegerTy WongAw HewittDa MackeyCl SimpsonN PfeifferO PärssinenPn BairdV VitartN AminCm Van DuijnJe Bailey-wilsonTl YoungS-m SawD StambolianS MacgregorJa GuggenheimJy TungCj HammondCcw Klaversubject
0301 basic medicineAdultMaleCell typeResearchInstitutes_Networks_Beacons/MICRAIn silicotaittovirheetGenome-wide association studyRetinal Pigment EpitheliumBiologyBlindnessPolymorphism Single NucleotideSensory disorders Donders Center for Medical Neuroscience [Radboudumc 12]ArticleRetinaWhite People03 medical and health sciencesHIGH-GRADE MYOPIA ; RETINAL-PIGMENT EPITHELIUM ; SEROTONIN PATHWAY GENES ; FORM-DEPRIVATION MYOPIA ; COMMON VARIANTS ; OCULAR GROWTH ; RETINITIS-PIGMENTOSA ; GENOTYPE IMPUTATION ; MISSENSE MUTATIONS ; DOPAMINE-RECEPTORSAsian Peoplerefractive errorsRetinitis pigmentosaGeneticsmedicineMyopiaJournal ArticleHumansGenetic Predisposition to Disease610 Medicine & healthRegulation of gene expressionRetinaRetinal pigment epitheliummedicine.diseaseRefractive Errors030104 developmental biologymedicine.anatomical_structureManchester Institute for Collaborative Research on AgeingGene Expression Regulationgenetic factorsEye disorderFemalesense organsgeneettiset tekijätNeuroscienceGenome-Wide Association StudySignal Transductiondescription
Skin affections after sulfur mustard (SM) exposure include erythema, blister formation and severe inflammation. An antidote or specific therapy does not exist. Anti-inflammatory compounds as well as substances counteracting SM-induced cell death are under investigation. In this study, we investigated the benzylisoquinoline alkaloide berberine (BER), a metabolite in plants like berberis vulgaris, which is used as herbal pharmaceutical in Asian countries, against SM toxicity using a well-established in vitro approach. Keratinocyte (HaCaT) mono-cultures (MoC) or HaCaT/THP-1 co-cultures (CoC) were challenged with 100, 200 or 300 mM SM for 1 h. Post-exposure, both MoC and CoC were treated with 10, 30 or 50 mu M BER for 24 h. At that time, supernatants were collected and analyzed both for interleukine (IL) 6 and 8 levels and for content of adenylate-kinase (AK) as surrogate marker for cell necrosis. Cells were lysed and nucleosome formation as marker for late apoptosis was assessed. In parallel, AK in cells was determined for normalization purposes. BER treatment did not influence necrosis, but significantly decreased apoptosis. Anti-inflammatory effects were moderate, but also significant, primarily in CoC. Overall, BER has protective effects against SM toxicity in vitro. Whether this holds true should be evaluated in future in vivo studies.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2018-06-01 |