6533b832fe1ef96bd129ae58
RESEARCH PRODUCT
More accuracy to the EROD measurements—The resorufin fluorescence differs between species and individuals
Eeva-riikka VehniäinenEija SchultzJanne A. IhalainenAimo OikariHeli Lehtivuorisubject
S9 fractionHealth Toxicology and MutagenesisAquatic ScienceFluorescence/dk/atira/pure/sustainabledevelopmentgoals/life_below_waterSpecies SpecificityOxidoreductaseCytochrome P-450 CYP1A1Ethoxyresorufin O-DeethylaseAnimalsSDG 14 - Life Below Waterchemistry.chemical_classificationEROD activityChromatographyChemistryEthoxyresorufin-O-deethylasefluoresenssiFishesta1182Reproducibility of ResultsFluorescenceEnzyme ActivationStandard curveS9 fractionResorufinBiomarkersWater Pollutants ChemicalEnvironmental Monitoringdescription
Ethoxyresorufin-O-deethylase (EROD) activity is a biomarker of exposure to planar aromatic hydrocarbons, and it is often measured from the S9 fraction. The effect of the liver S9 fraction of seven boreal freshwater fish species on the fluorescence of resorufin was studied. The S9 fractions diminished resorufin fluorescence by 40–80%, and there were large differences between species. Thus, using a resorufin standard curve without the S9 fraction leads to a large underestimation of the EROD activity. Therefore a microwell plate EROD method was developed that takes into account the effect of each sample on resorufin fluorescence. At least two mechanisms were involved in the decrease of the fluorescence: opaqueness of the sample, and enzymes (DT-diaphorase and plausibly NADPH-CYP450 oxidoreductase) that reduce resorufin to a non-fluorescent form. peerReviewed
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2012-07-15 | Aquatic Toxicology |