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RESEARCH PRODUCT
Endothelialization and Anticoagulation Potential of Surface-Modified PET Intended for Vascular Applications.
Hugo ThienpontCharles James KirkpatrickHeidi OttevaereDavid SchaubroeckPeter DubruelElena Diana GiolRonald E. UngerSandra Van VlierbergheSandra Van Vlierberghesubject
LipopolysaccharidesPolymers and PlasticsPoly(ethylene terephthalate)Gene ExpressionBiocompatible Materials02 engineering and technology01 natural sciencesGelatinendothelializationchemistry.chemical_compoundCoatingPolyethylene terephthalateMaterials Chemistrychemistry.chemical_classificationPolyethylene TerephthalatesSurface modifiedhemocompatibility021001 nanoscience & nanotechnologyPlatelet Endothelial Cell Adhesion Molecule-10210 nano-technologyE-Selectinbiotechnologyendotoxin contentazide photograftingAzidesfood.ingredientMaterials scienceBiocompatibilityCell SurvivalSurface PropertiesBioengineeringengineering.material010402 general chemistryBiomaterialsfoodvon Willebrand FactorHuman Umbilical Vein Endothelial CellsHumansTissue EngineeringBiomoleculeAnticoagulants0104 chemical sciencesBlood Vessel ProsthesischemistryengineeringSurface modificationBlood VesselsGelatinAzideBiomarkersBiomedical engineeringdescription
In vascular tissue engineering, great attention is paid to the immobilization of biomolecules onto synthetic grafts to increase bio- and hemocompatibility-two critical milestones in the field. The surface modification field of poly(ethylene terephthalate) (PET), a well-known vascular-graft material, is matured and oversaturated. Nevertheless, most developed methods are laborious multistep procedures generally accompanied by coating instability or toxicity issues. Herein, a straightforward surface modification procedure is presented engineered to simultaneously promote surface endothelialization and anticoagulation properties via the covalent immobilization of gelatin through a photoactivated azide derivative. A complete physicochemical characterization and biological study including cytotoxicity and endotoxin testing are performed. In addition, biocompatibility toward small (diameter ≤ 6 mm) and/or large caliber (diameter ≥ 6 mm) vessels is assessed by micro- and macrovascular endothelial cell assays. Superior bio- and hemocompatibility properties are seen for the gelatin-covalently modified PET surfaces compared to the conventional surface-modification procedures based on physisorption.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2018-07-01 | Macromolecular bioscience |