6533b833fe1ef96bd129b7e1
RESEARCH PRODUCT
Trapping of 27 bp–8 kbp DNA and immobilization of thiol-modified DNA using dielectrophoresis
Sampo TuukkanenMarcus RinkiöJ. Jussi ToppariAnton KuzykHannu HäkkinenVesa P. HytönenPäivi TörmäEinari A. Niskanensubject
Materials scienceFOS: Physical sciencesBioengineeringTrappingCondensed Matter - Soft Condensed Matterlaw.inventionchemistry.chemical_compoundConfocal microscopylawGeneral Materials SciencePhysics - Biological PhysicsElectrical and Electronic EngineeringNanoscopic scalechemistry.chemical_classificationMechanical EngineeringBiomolecules (q-bio.BM)General ChemistryDielectrophoresisCondensed Matter - Other Condensed MatterQuantitative Biology - BiomoleculeschemistryBiological Physics (physics.bio-ph)Mechanics of MaterialsFOS: Biological sciencesElectrodeThiolBiophysicsSoft Condensed Matter (cond-mat.soft)Density functional theoryDNAOther Condensed Matter (cond-mat.other)description
Dielectrophoretic trapping of six different DNA fragments, sizes varying from the 27 to 8416 bp, has been studied using confocal microscopy. The effect of the DNA length and the size of the constriction between nanoscale fingertip electrodes on the trapping efficiency have been investigated. Using finite element method simulations in conjunction with the analysis of the experimental data, the polarizabilities of the different size DNA fragments have been calculated for different frequencies. Also the immobilization of trapped hexanethiol- and DTPA-modified 140 nm long DNA to the end of gold nanoelectrodes was experimentally quantified and the observations were supported by density functional theory calculations.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2006-09-18 | Nanotechnology |