6533b833fe1ef96bd129b9da
RESEARCH PRODUCT
Human papillomavirus infection requires cell surface heparan sulfate.
Frank SchäferMartin SappLuise FlorinRolf E. StreeckTzenan Giroglousubject
ImmunologyIntegrinIntegrin alpha6Microbiologychemistry.chemical_compoundSulfationAntigens CDVirologymedicineAnimalsHumansChondroitin sulfateReceptorNeural Cell Adhesion MoleculesPapillomaviridaeAntiserumHeparinaseMembrane GlycoproteinsbiologyHeparinVirionHeparan sulfateHeparinMolecular biologyVirus-Cell InteractionschemistryInsect ScienceCOS Cellsbiology.proteinHeparitin SulfateLeukocyte L1 Antigen Complexmedicine.drugdescription
ABSTRACT Using pseudoinfection of cell lines, we demonstrate that cell surface heparan sulfate is required for infection by human papillomavirus type 16 (HPV-16) and HPV-33 pseudovirions. Pseudoinfection was inhibited by heparin but not dermatan or chondroitin sulfate, reduced by reducing the level of surface sulfation, and abolished by heparinase treatment. Carboxy-terminally deleted HPV-33 virus-like particles still bound efficiently to heparin. The kinetics of postattachment neutralization by antiserum or heparin indicated that pseudovirions were shifted on the cell surface from a heparin-sensitive into a heparin-resistant mode of binding, possibly involving a secondary receptor. Alpha-6 integrin is not a receptor for HPV-33 pseudoinfection.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2001-02-01 | Journal of virology |