6533b833fe1ef96bd129c0c9
RESEARCH PRODUCT
Chromatographic peak profile of ionogenic analytes upon elution with unbuffered eluents
K.k. UngerA.a. Kurganovsubject
chemistry.chemical_classificationAnalyteChromatographyElutionCarboxylic acidOrganic ChemistryAnalytical chemistryGeneral MedicineReversed-phase chromatographyBiochemistryHigh-performance liquid chromatographyAnalytical ChemistryDissociation constantchemistry.chemical_compoundAdsorptionchemistryBenzoic aciddescription
Experimental proof of the model was carried out for the separation of benzoic acid on a LiChrospher RP-18 column. The observed chromatographic peak profile coincides with the peak profile predicted by the model. The retention times of the dissociated and non-dissociated species determined by using an appropriate fitting procedure were 2.2 and 4.3 min, respectively. Based on these values, a theoretical peak profile was calculated, demonstrating a close agreement with the experimentally observed peak profile. An unexpectedly large difference was found for the retention times of the dissociated and non-dissociated species calculated by a fitting procedure and those experimentally measured on the elution of benzoic acid with buffered eluents. A possible explanation for this difference is a contribution of the ion-exchange adsorption on the silica matrix.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1998-02-01 | Journal of Chromatography A |