6533b833fe1ef96bd129c12c
RESEARCH PRODUCT
Characterization of liver cytokeratin as a major target antigen of anti-SLA antibodies.
A. KyriatsoulisMichael MannsAw LohseG. GerkenB. WächterK H Meyer Zum Büschenfeldesubject
Anti-nuclear antibodyImmunoblottingFluorescent Antibody TechniqueEnzyme-Linked Immunosorbent AssayImmunofluorescenceChromatography AffinityCytokeratinAntigenAffinity chromatographymedicineHumansAntigensAutoantibodiesHepatologymedicine.diagnostic_testbiologyfungiS100 ProteinsAntibodies MonoclonalVirologyPrimary and secondary antibodiesImmunohistochemistryLiverMonoclonalbiology.proteinKeratinsElectrophoresis Polyacrylamide GelAntibodydescription
Abstract Anti-SLA antibodies characterize a newly defined subgroup of patients with autoimmune chronic active hepatitis. The aim of the present study was the immunochemical characterization of the target antigen(s) of anti-SLA antibodies. Anti-SLA-positive sera were found to contain high titres of anti-cytokeratin antibodies. In immunoblotting analyses with 100 000 × g supernatants of human liver homogenates (S-100) these sera recognized various proteins with a molecular mass of 40–60 kDa. These proteins were also recognized by monoclonal anti-cytokeratin antibodies. Two-dimensional co-electrophoresis and immunoblotting analysis of S-100 and liver cytokeratins showed that anti-SLA antibodies were primarily directed against cytokeratins, particularly against liver cytokeratin types 8 and 18. This was supported by affinity chromatography, immunofluorescence and absorption methods using anti-SLA sera.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1990-09-01 | Journal of hepatology |