6533b833fe1ef96bd129c7f1

RESEARCH PRODUCT

Reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) as a user-friendly system to detect SARS-CoV-2 infection: a multicentric study

Stracquadanio StefanoDi Gaudio FrancescaGiunta ElenaFalliti GiuseppeCaruso DanielePinzone CarmeloNoto PatriziaDi Naso ConcettinaGarozzo Sebastiano FabioAmodeo AndreaCinà DianaCardillo Maria ConcettaZappalà SimonaConsoli AlessandroStefani Stefania

subject

Multicentric studyRT-LAMP.Diagnostic powerReverse Transcriptase Polymerase Chain ReactionSARS-CoV-2COVID-19RNA-Directed DNA PolymeraseSensitivity and SpecificityMolecular Diagnostic TechniquesHumansRNA ViralRNAViralNucleic Acid Amplification Techniques

description

Although reverse transcriptase quantitative PCR remains the gold standard to perform viral detection, reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) is already used to perform diagnosis of various infections. This work reports the results of a multicentric study performed in Sicily to evaluate the diagnostic power of an RT-LAMP kit for the diagnosis of SARS-CoV-2 infection on a total of 551 samples collected in January and February 2021, revealing sensitivity, specificity, accuracy, positive and negative predictive values ≥95%. Our results suggest the potential employment of this kit as a screening test to be used where fast and reliable results are demanded without the need for expensive instruments and highly-skilled personnel.

yearjournalcountryeditionlanguage
2021-11-16
https://hdl.handle.net/20.500.11769/540503