6533b833fe1ef96bd129ca5d
RESEARCH PRODUCT
Dihydrodiol Dehydrogenase: An Important Enzyme in Dihydrodiol-Epoxide Pathway — Mediated Benzo(A)Pyrene Mutagenicity
K. VogelHansruedi GlattKarl-ludwig PlattFranz OeschPhilip Bentleysubject
chemistry.chemical_classificationchemistry.chemical_compoundEnzymeEpoxide HydratasechemistryBenzo(a)pyreneBiochemistrypolycyclic compoundsEpoxidePyreneMonooxygenaseDihydrodiol dehydrogenaseProtective systemdescription
Benzo(a)pyrene is metabolized to two major groups of mutagenically reactive metabolites: Monofunctional epoxides and dihydrodiol-epoxides. Various monooxygenase forms catalyze the various pathways at very different rates. In metabolic situations where the contribution by dihydrodiol-epoxides is small, epoxide hydratase represents a very efficient protective system. However, in situations where the mutagenic effect is predominately due to dihydrodiol-epoxide, the effect of epoxide hydratase is complicated and weak. We have now obtained evidence that a dihydrodiol dehydrogenase represents an efficient protective system in the latter situation. The enyzme was purified to homogeneity and the pure enyzme added to systems generating mutagenically active benzo(a)pyrene metabolites by the presence of various monooxygenase forms. In situations where epoxide hydratase had only weak effects, dihydrodiol dehydrogenase afforded efficient protection and vice-versa.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1978-01-01 |