Transient Transformation of A. thaliana Seedlings by Vacuum Infiltration

6533b834fe1ef96bd129cb0d

RESEARCH PRODUCT

Transient Transformation of A. thaliana Seedlings by Vacuum Infiltration

César Bernat-silvestre Vanessa De Sousa Vieira María Jesús Marcote Fernando Aniento Judit Sánchez-simarro

subject

0106 biological sciences 0301 basic medicine Expression vector biology Agrobacterium Chemistry Confocal Mutant Agrobacterium tumefaciens medicine.disease biology.organism_classification 01 natural sciences Fluorescence Cell biology 03 medical and health sciences 030104 developmental biology Arabidopsis medicine Infiltration (medical)010606 plant biology & botany

description

Transient expression in Arabidopsis thaliana seedlings allows fast expression of fluorescent markers for different subcellular compartments. This protocol describes a transient transformation assay with five-day-old seedlings using Agrobacterium tumefaciens-mediated vacuum infiltration. Three days after infiltration of the Agrobacterium containing an expression vector for a fluorescent marker of interest, cotyledon cells expressing the fluorescent protein can be imaged in a confocal microscope. This assay allows high-throughput screening of new constructs and the study of the localization of a large number of subcellular markers in Arabidopsis seedlings including wild-type, stable over-expressing and mutant lines.

year	journal	country	edition	language
2020-11-12

https://doi.org/10.1007/978-1-0716-0880-7_6

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