6533b834fe1ef96bd129cb47
RESEARCH PRODUCT
A combined molecular typing approach does not discriminate Legionella pneumophila serogroup 1 strains of a predominant sequence-based type in Palermo, Italy
Celestino BonuraAnna GiammancoAntonietta VellaAlfredo ChiariniSantina BelfioreCaterina Mamminasubject
Settore MED/07 - Microbiologia E Microbiologia ClinicaGenotypeEpidemiologyLegionella pneumophila Molecular typing Epidemiology SBT PFGEMolecular typingLegionella pneumophilaLegionella pneumophilaMicrobiologylcsh:Infectious and parasitic diseasesMolecular typingType (biology)Pulsed-field gel electrophoresisHumansSBTlcsh:RC109-216TypingLegionella pneumophila Serogroup 1Sequence (medicine)biologylcsh:Public aspects of medicineSputumPublic Health Environmental and Occupational Healthlcsh:RA1-1270PFGEGeneral Medicinebiology.organism_classificationCombined approachElectrophoresis Gel Pulsed-FieldInfectious DiseasesItalyLegionnaires' DiseaseWater MicrobiologySequence Analysisdescription
Summary: The sequence-based type 1,4,3,1,1,1 of Legionella pneumophila sg.1 is predominant in the Palermo city environment since several years. In this study, extended sequence-based typing and pulsed field gel electrophoresis were used in a combined approach in the aim to enhance discriminatory power of the molecular typing procedures. However, probably due to a common environmental reservoir and genetic stability, most of the strains circulating in the geographic area under study belong to the same clone and are, consequently, indistinguishable by molecular typing. Investigations of clinical cases and tracing to their environmental source require caution and support from sound epidemiological data. Keywords: Legionella pneumophila, Molecular typing, Epidemiology, SBT, PFGE
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2009-07-07 | Journal of Infection and Public Health |