6533b834fe1ef96bd129cdc7

RESEARCH PRODUCT

TWIST and p-Akt immunoexpression in normal oral epithelium, oral dysplasia and in oral squamous cell carcinoma

Flávia-sirotheau Corrêa PontesFernanda-paula YamamotoBruno-santos De Freitas SilvaHélder Antônio Rebelo PontesFelipe Paiva FonsecaSérgio Elias-vieira CuryDécio-dos Santos Pinto-junior

subject

MalePathologymedicine.medical_specialtyEpitheliumTwist transcription factormedicineHumansGeneral DentistryMouth neoplasmOral DysplasiaOral Medicine and PathologyImmunoperoxidasebusiness.industryTwist-Related Protein 1CancerMiddle Aged:CIENCIAS MÉDICAS [UNESCO]medicine.diseaseEpitheliumstomatognathic diseasesmedicine.anatomical_structureOtorhinolaryngologyDysplasiaUNESCO::CIENCIAS MÉDICASCarcinoma Squamous CellImmunohistochemistryFemaleMouth NeoplasmsResearch-ArticleSurgeryLeukoplakia OralbusinessProto-Oncogene Proteins c-akt

description

Objectives: The aim of this study was to evaluate the immunoexpression of TWIST and p-Akt proteins in oral leukoplakia (OL) and oral squamous cell carcinoma (OSCC), correlating their expressions with the histological features of the lesions. Study design: Immunohistochemical studies were carried out on 10 normal oral epithelium, 30 OL and 20 OSCC formalin-fixed, paraffin-embedded tissue samples. Immunoperoxidase reactions for TWIST and p-Akt proteins were applied on the specimens and the positivity of the reactions was calculated for 1000 epithelial cells. Results: Kruskal-Wallis and Dunn’s post tests revealed a significant difference in TWIST and p-Akt immunoexpression among normal oral mucosa, OL and OSCC. In addition, a significant positive correlation was found between TWIST and p-Akt expressions according to the Pearson’s correlation test. Conclusions: The results obtained in the current study suggest that TWIST and p-Akt may participate of the multi-step process of oral carcinogenesis since its early stages. Key words: Oral cancer, oral leukoplakia, dysplasia, immunohistochemistry.

yearjournalcountryeditionlanguage
2010-10-06Medicina Oral Patología Oral y Cirugia Bucal
https://doi.org/10.4317/medoral.17344