6533b834fe1ef96bd129d77e
RESEARCH PRODUCT
Cloning of Clostridium difficile toxin B gene and demonstration of high N-terminal homology between toxin A and B.
C. Von Eichel-streiberJ SchulzeS. SartingenRita Laufenberg-feldmannMarkus Sauerbornsubject
Microbiology (medical)DNA BacterialImmunologyBacterial ToxinsMolecular Sequence DataRestriction MappingClostridium difficile toxin AClostridium difficile toxin BMolecular cloningBiologyCross ReactionsHomology (biology)Restriction mapBacterial ProteinsSequence Homology Nucleic AcidImmunology and AllergyAmino Acid SequenceCloning MolecularPeptide sequenceGeneticsBase SequenceClostridioides difficileNucleic acid sequenceGeneral MedicineMolecular biologyAntibodies BacterialOpen reading frameGenes BacterialDNA Probesdescription
High titered Clostridium sordellii lethal toxin antiserum, cross-reactive with C. difficile cytotoxin B (ToxB), was used to isolate toxB fragments from a C. difficile expression library. Recombinant clones containing toxB fragments of the 5' and 3' end were isolate. A 2.5-kb HincII fragment of chromosomal DNA overlaps both groups of clones. A partial restriction map of the total toxB gene is presented. The gene is positioned upstream of utxA and toxA, toxB has a size of 6.9 kb, corresponding to a 250-kDa polypeptide. A partial sequence of the 5' end of toxB was determined. The sequence contains 398 bp upstream of toxB with a putative Shine-Dalgarno box (AGGAGA) and 609 bp of the toxB open reading frame. The N-terminal 203 amino acids of ToxB were compared with the N-terminal amino acids of the enterotoxin A (ToxA). A homology of 64% of the residues was detected, which proves the relatedness of ToxA and ToxB of C. difficile.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1990-12-01 | Medical microbiology and immunology |