6533b834fe1ef96bd129d8b5
RESEARCH PRODUCT
A single primer pair gives a specific ortholog amplicon in a wide range of Cyanobacteria and plastid-bearing organisms: applicability in inventory of reference material from collections and phylogenetic analysis.
C RoyoEva BarrenoE. M. Del CampoA. Del HoyoRaquel ÁLvarezLeonardo M. Casanosubject
DNA BacterialDNA PlantGlaucophytaCyanobacteriaPhylogeneticsChlorophytaGeneticsCryptophytaGlaucophytaPlastidsPlastidMolecular BiologyEcology Evolution Behavior and SystematicsPhylogenyDNA PrimersGeneticsPhylogenetic treebiologyStreptophytaSequence Analysis DNARibosomal RNAAmpliconbiology.organism_classificationRNA Ribosomal 23SEvolutionary biologyRhodophytaStreptophytaStramenopilesdescription
The scarcity of universally applied molecular markers for algae has resulted in the development of multiple, independent and not easily comparable systems. The goal of this work is to increase the number of available molecular markers and to generate easily comparable systems. Thereby, we have designed a primer pair capable of amplifying a broad range of organisms: Cyanobacteria, Chlorophyta, Chlorarachniophyta, Cryptophyta, Euglenida, Glaucophyta, Rhodophyta, Stramenopiles and Streptophyta including plants. This primer pair can amplify a portion of the 23S rRNA gene with sufficient variability to identify reference material form collections across a broad range of taxa and perform phylogenetic studies alongside other available markers.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2010-12-01 | Molecular phylogenetics and evolution |