6533b834fe1ef96bd129d8cd

RESEARCH PRODUCT

Transfection of lipoma cells with papilloma bovine virus subgenomic fragment.

Lorraine RacineOlga HausC. Turc-carelJean-pierre ZahndPrudent PadieuMaryvonne Moisant

subject

virusesCellEndoplasmic ReticulumTransfectionVirusPlasmidotorhinolaryngologic diseasesmedicineTumor Cells CulturedHumansBovine papillomavirusSubgenomic mRNABovine papillomavirus 1Cell Line TransformedChromosome AberrationsbiologyMusclesCell DifferentiationCell BiologyTransfectionFibroblastsbiology.organism_classificationmedicine.diseaseCell Transformation ViralVirologyClone CellsMicroscopy Electronmedicine.anatomical_structureAdipose TissueCell culturePapillomaLipomaCell Division

description

Abstract Lipoma cells with consistent chromosomal aberration have been transfected with plasmids carrying papilloma bovine virus subgenomic fragment (PBV 69). The succesful transformation of the cells was ascerted on the changed growth pattern of the cells in liquid medium, colony formation in soft agar and modified cell appearrance in electron microscopy; transfection with PBV 69 has not been, however, sufficient to immortalize lipoma cells.

10.1016/0309-1651(91)90083-uhttps://pubmed.ncbi.nlm.nih.gov/1848483