6533b834fe1ef96bd129e09b
RESEARCH PRODUCT
Development of HPLC methods for the purification and analysis of plasma membrane glycoproteins.
Doris RenauerH U GierlichK K Ungersubject
Membrane GlycoproteinsChromatographyChemistryHealth Toxicology and MutagenesisHydrophilic interaction chromatographyCell MembraneIon chromatographyPublic Health Environmental and Occupational HealthReversed-phase chromatographyChromatography Ion ExchangeHigh-performance liquid chromatographyDisplacement chromatographyChromatography AffinityAffinity chromatographyEvaluation Studies as TopicProtein purificationChromatography GelHumansChromatography columnChromatography High Pressure LiquidResearch ArticleCell Line Transformeddescription
High resolution HPLC techniques such as affinity chromatography (AC), ion exchange chromatography (IEC), and size exclusion chromatography (SEC) were used successfully for separations of hydrophobic plasma membrane glycoproteins. We have tested a lot of commercially available columns for IEC and SEC and performed the purification of the crude plasma membrane extract with the most suitable columns. By using immobilized ligands with different specificities and sequential affinity chromatography, it is possible to obtain a preliminary structural characterization of the interesting carbohydrate residues of membrane glycoproteins.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1990-08-01 | Environmental Health Perspectives |