6533b834fe1ef96bd129e241

RESEARCH PRODUCT

Localization of amplified CAD genes on rearranged chromosomes of Chinese hamster cells

Stefania BonattiP. CavolinaAngelo AbbondandoloMariangela MieleAldo Di LeonardoCinzia AgneseLaura Ottaggio

subject

Geneticseducation.field_of_studybiologyMarker chromosomeClinical BiochemistryBiomedical EngineeringBioengineeringCell Biologybiology.organism_classificationMolecular biologyNucleolar Organizer RegionChinese hamsterDicentric chromosomeHomologous chromosomeeducationSmall supernumerary marker chromosomeChromosome 22Homogeneously Staining RegionBiotechnology

description

Chinese hamster cell lines carrying an amplified CAD region were selected from V79,B7 cells by their resistance to N-phosphonacetyl-L-aspartate (PALA). In one of the selected cell lines, SP PALA (inf1) (supR) L, an acrocentric chromosome with abnormally elongated q arms was identified as a marker for the PALA-resistant phenotype. The marker chromosome carried a homogeneously staining region close to a telomeric nucleolar organizer region. In the same region, localization of amplified CAD sequences was demonstrated by in situ hybridization. The marker chromosome was found to undergo extensive rearrangements. In particular, dicentric chromosomes, occurring with an unusually high incidence, were found to originate from end-fusion of two homologous marker chromosomes.

yearjournalcountryeditionlanguage
2012-02-24Cytotechnology
https://doi.org/10.1007/bf00351118