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RESEARCH PRODUCT

Second generation sequencing of three STRs D3S1358, D12S391 and D21S11 in Danes and a new nomenclature for sequenced STR alleles

Sigrun DalsgaardNiels MorlingEszter RockenbauerClaus BørstingChiara GelardiChiara Gelardi

subject

GeneticsDenmarkSTR multiplex systemSingle-nucleotide polymorphismLocus (genetics)Sequence Analysis DNABiologyPathology and Forensic MedicineGene FrequencyTerminology as TopicGeneticsHumansStr typingAlleleAllele frequencyNomenclatureForensic geneticsMicrosatellite Repeats

description

Second generation sequencing (SGS) may revolutionize the field of forensic STR typing. Two of the essential requirements for implementation of an SGS based approach for forensic investigations are (1) establishment of adequate frequency databases and (2) adoption of a new STR nomenclature. We report the STR sequences and allele frequencies of three STR loci: D3S1358, D12S391 and D21S11 in 197 unrelated Danes. We used a new STR nomenclature that depicts the locus name used in forensic genetics, the length of the repeat region divided by the repeat length (typically 4 nucleotides) and detailed sequence information of possible sub-repeats and SNPs within the amplified fragment.

yearjournalcountryeditionlanguage
2014-09-01Forensic Science International: Genetics
https://doi.org/10.1016/j.fsigen.2014.04.016