6533b835fe1ef96bd129ec5c
RESEARCH PRODUCT
l-Glutamate receptor binding in bovine retina
Walter E. MüllerStephan H.j. Höckelsubject
PopulationGlutamic AcidReceptors Cell SurfaceBiologyInhibitory postsynaptic potentialRetinaCellular and Molecular NeuroscienceGlutamatesAnimalsCentrifugationBinding siteeducationeducation.field_of_studyDose-Response Relationship DrugSodiumGlutamate receptorGlutamate bindingGlutamic acidSensory SystemsReceptors NeurotransmitterDissociation constantOphthalmologyReceptors GlutamateBiochemistryCattleSubcellular Fractionsdescription
Using a centrifugation technique saturable specific [ 3 H]glutamate binding in bovine retina could be demonstrated. Scatchard analysis revealed only one population of binding sites with a dissociation constant of about 3 μ m and a maximal number of binding sites of about 0·2 pmol/mg retinal protein. Several glutamic acid analogues inhibit specific [ 3 H]glutamate binding in bovine retina with half-maximal inhibitory concentrations similar to those reported in other areas of the CNS. Specific [ 3 H]glutamate binding and sodium dependent synaptosomal uptake of glutamate are largely concentrated in the P2 fraction of bovine retina homogenates consisting of conventionally sized synaptosomes. The data suggest the presence of glutamate receptors in the bovine retina with a distinct regional distribution.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1982-07-01 | Experimental Eye Research |