6533b835fe1ef96bd129ec9a

RESEARCH PRODUCT

Sugar-induced stabilization of the monoolein Pn3m bicontinuous cubic phase during dehydration

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To explore the molecular mechanism of the protective function of sugars on cubic lipidic systems, the mesomorphic properties of the monoolein-water system, dehydrated in the presence of a series of sugars, have been studied by osmotic stress experiments. Two bicontinuous inverse cubic structures $(Pn3m$ and $\mathrm{Ia}3d)$ and a lamellar ${L}_{\ensuremath{\alpha}}$ phase form under dehydration in pure water. In sugar solutions, the $\mathrm{Pn}3m$ phase shows an extraordinary stability: as a function of sugar concentration, the lattice parameter decreases to very low values, but no phase transitions occur. Instead, the $\mathrm{Pn}3m$ to $\mathrm{Ia}3d$ phase transition is obtained by equilibrating the lipid phase with aqueous polymer solutions of increasing osmotic pressure. As a result, the pressure at which the phase transition occurs strongly depends on sugar concentration. The free-energy curves obtained from the osmotic-pressure unit-cell data show that the sugar exerts an additional stabilization on both the cubic phases. The analysis of the structural parameters indicates that sugars alter the interface geometry. We suggest that a consequent release of stretching contributions in the chain packing or a reduction of the inhomogeneity in molecular splay mainly stabilize the $\mathrm{Pn}3m$ phase and prevent the transition to the $\mathrm{Ia}3d$ phase on dehydration.