Ekstracelulārā matriksa sintēzes un degradācijas loma ādas dzīšanā un tās analīze biotehnoloģiski veidotos ādas audos

6533b835fe1ef96bd129eea0

RESEARCH PRODUCT

Ekstracelulārā matriksa sintēzes un degradācijas loma ādas dzīšanā un tās analīze biotehnoloģiski veidotos ādas audos

Liene Pirtniece

subject

ekstracelulārais matrikss eksudāti brūču dzīšana Bioloģija kokultūras 3D ādas audu modelis

description

Darba mērķis ir izstrādāt ādas audu modeli in vitro, balstītu uz ādas šūnu kokultūru kultivēšanu trīsdimensionālā fibrīna matriksā, kā arī noteikt izveidotajos audos ekstracelulārā matriksa (ECM) komponentu sintēzi un degradāciju. Papildus tika ievākti arī hronisku brūču eksudātu paraugi, kuriem tika veikta dažādu – ar brūču dzīšanu saistītu marķieru analīze. Mērķa sasniegšanai tika izmantotas šūnu, molekulārās bioloģijas un imunoloģijas metodes. Darbā iegūtie rezultāti parāda ECM komponentu, to degradējošo enzīmu un augšanas faktoru produkcijas izmaiņas audu kultivēšanas laikā un ļauj izvēlēties optimālu ādas audu modeļsistēmas veidošanas un kultivēšanas metodiku. Darbs tika izstrādāts Paula Stradiņa Klīniskās universitātes slimnīcas Šūnu transplantācijas centra laboratorijā, Latvijas Universitātes Mikrobioloģijas un biotehnoloģijas katedrā un Latvijas Universitātes Bioanalītisko un biodozimetrijas metožu laboratorijā no 2013. gada septembra līdz 2015. gada maijam

year	journal	country	edition	language
2015-01-01

https://dspace.lu.lv/dspace/handle/7/29460

PEPhysical Sciences and Engineering
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